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cd44 ebioscience clone im7 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd44 ebioscience clone im7 antibody
    Cd44 Ebioscience Clone Im7 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+(ebioscience%2C+clone%3A+im7)+antibody/anti+cd44/pm37563311-558-23-24
    Average 90 stars, based on 1 article reviews
    cd44 ebioscience clone im7 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Control:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Injection:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Gene Expression:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Staining:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Virus:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Plasmid Preparation:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    CRISPR:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Recombinant:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    SYBR Green Assay:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Infection:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Protease Inhibitor:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Isolation:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Cell Isolation:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Activation Assay:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Amplification:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).

    Software:

    Article Title: Preserved suppressive function despite loss of Foxp3: insights into the identity of regulatory T cells
    Article Snippet: Anti-CD44 (clone: IM7), anti-CD62L (clone: MEL-14), anti-Foxp3 (clone: FJK-16s), anti-CD45.2 (clone: 104), anti- and anti-GITR (clone: DTA-1) were purchased from eBioscience. were purchased from Biolegend.

    Article Title: Efficient and Selective Biosynthesis of a Precursor-Directed FK506 Analogue: Paving the Way for Click Chemistry.
    Article Snippet: Clones and suppliers of mAbs and reagents were: anti-CD4 PE (GK1.5) and anti-CD44 FITC (IM7), from eBioscience (USA); Aqua reagent for live/dead discrimination, from BioLegend (USA); and CellTrace Violet Cell Proliferation kit, from Life Technologies.

    Article Title: Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.
    Article Snippet: The following antibodies were purchased from eBioscience: Anti-mouse CD11b-APC-e780 (clone M1/70, catalog number 47-0112-82, dilution 1:100), CD44-APC-eF780 (clone IM7, catalog number 47-0441-80, dilution 1:200), CD45-APC-eF780 (clone 30-F11, catalog number 47-0451-82, dilution 1:100), CD45-FITC (clone 30-F11, catalog number 11-0451-82, dilution 1:500), CD8α-AF700 (clone 53-6.7, catalog number 56-0081-82, dilution 1:100), Foxp3PerCP-Cy5.5 (clone FJK-16s, catalognumber45-5773-82, dilution 1:100), GATA3-eF660 (cloneTWAJ, catalognumber 50-9966-42, dilution 1:20), IL-13-PE (clone eBio13A, catalog number 12-7133-82, dilution 1:100) and RORγt-PE (clone AFKJS-9, catalog number 12-6988-82, dilution 1:100) and ST2(IL33R)-Biotin (clone RMST2-2, catalog number 13-9335-82, dilution 1:100).

    Article Title: Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection.
    Article Snippet: Pathogen-induced memory Tfh cells are important to maintain high-affinity antibodies against pathogens.. We have now discovered Tfh cells with a similar memory phenotype (MP) that develop in pathogen-free conditions.. These MP Tfh cells are similar to pathogen-induced memory Tfh in both phenotype and function.

    Article Title: Metabolic fitness of NAC1-deficient Tregs in the tumor microenvironment fuels tumor growth.
    Article Snippet: The following antibodies against mouse proteins were used: anti-CD45 (BioLegend clone 30-F11), anti-CD3ε (eBioscience clone 17A2), antiCD4 (eBioscience clone RM4-5), anti-CD8α (eBioscience clone 53.6.7), anti-CD44 (eBioscience clone IM7), anti-CD4 (eBioscience clone GK1.5), anti-CD25 (BioLegend clone 3C7), anti-CD36 (BioLegend clone HM36), anti-FoxP3 (BioLegend clone MF-14), anti-GFP/YFP (BioLegend clone FM264G), anti-Thy1.2 (BioLegend clone 30-H12), anti–PGC-1α (Novus Biologicals, Bio-Techne; clone NBP1-04676, polyclonal), and anti–Granzyme B (BioLegend clone GB11).

    Article Title: A microRNA-regulated transcriptional state defines intratumoral CD8 + T cells that respond to immunotherapy
    Article Snippet: Pacific Blue anti-mouse CD44 Antibody (Clone #IM7) , eBioscience , Cat#103019; RRID:AB_493682.

    Article Title: CD44 Participates to Extramedullary Haematopoiesis Onset by Mediating the Interplay Between Monocytes and Haematopoietic Stem Cells in Myelofibrosis
    Article Snippet: For in vitro extravasation assay the following antibodies and corresponding isotype controls were used: monoclonal anti‐CD44 antibody (clone IM7, cat. #14‐0441‐82), rat IgG2a kappa Isotype Control as control IgG for the monoclonal anti‐CD44 antibody (clone eBR2a, cat. #14‐4321‐82; both from eBioscience, Invitrogen, ThermoFisher, Waltham, Massachussets, USA); Polyclonal anti‐CD44 antibody (clone HCAM DF1485, cat. #sc‐7297), anti‐αvβ3 antibody (clone 23C6, cat. #sc‐7312), normal mouse IgG as control IgG for the polyclonal anti‐CD44 and anti‐αvβ3 antibodies (cat. #sc‐2025, all from Santa Cruz Biotechnology Inc., Dallas, Texas, USA); anti‐α4β1 antibody, InVivoMAb anti‐mouse/human VLA‐4 (CD49d) (clone PS/2, cat. #BE0071), InVivoMAb rat IgG2b isotype control as control IgG for the anti‐α4β1 antibody (clone LTF‐2, cat. #BE0090, all from BioXCell, Lebanon, New Hampshire, USA); anti‐human OPN Antibody (cat. AF1433, Biotechne, R&D systems, Minnesota, USA); Hyaluronan inhibitor (cat. #AS‐62622, Anaspec, California, USA).



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    antibodies cd44 ebioscience clone im7 - by Bioz Stars, 2026-09
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    90
    Thermo Fisher cd44 pc7 ebioscience clone im7 antibody
    Cd44 Pc7 Ebioscience Clone Im7 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+(ebioscience%2C+clone%3A+im7)+antibody/anti+cd44/pm24905804-60-30-32
    Average 90 stars, based on 1 article reviews
    cd44 pc7 ebioscience clone im7 antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher cd44 (ebioscience, clone: im7) antibody
    Cd44 (Ebioscience, Clone: Im7) Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+(ebioscience%2C+clone%3A+im7)+antibody/anti+cd44/10__1007_slash_978___1___60761___720___4-5817-118-119
    Average 90 stars, based on 1 article reviews
    cd44 (ebioscience, clone: im7) antibody - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

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